Genotype-free demultiplexing of pooled single-cell RNA-seq
نویسندگان
چکیده
منابع مشابه
Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens
Genetic screens help infer gene function in mammalian cells, but it has remained difficult to assay complex phenotypes-such as transcriptional profiles-at scale. Here, we develop Perturb-seq, combining single-cell RNA sequencing (RNA-seq) and clustered regularly interspaced short palindromic repeats (CRISPR)-based perturbations to perform many such assays in a pool. We demonstrate Perturb-seq b...
متن کاملDissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq
In multicellular organisms, dedicated regulatory circuits control cell type diversity and responses. The crosstalk and redundancies within these circuits and substantial cellular heterogeneity pose a major research challenge. Here, we present CRISP-seq, an integrated method for massively parallel single-cell RNA sequencing (RNA-seq) and clustered regularly interspaced short palindromic repeats ...
متن کاملI-42: Origins and Differentiation of Somatic Progenitors of The Mammalian Gonad Revealed by Single Cell RNA-Seq
Background - MaterialsAndMethods N;Results N;Conclusion N;
متن کاملDetecting heterogeneity in single-cell RNA-Seq
21 Single-cell RNA-Sequencing (scRNA-Seq) is a cutting edge technology that enables the 22 understanding of biological processes at an unprecedentedly high resolution. However, 23 well suited bioinformatics tools to analyze the data generated from this new technology 24 are still lacking. Here we have investigated the performance of non-negative matrix 25 factorization (NMF) method to analyze a...
متن کاملSingle-cell RNA-Seq unveils tumor microenvironment
Single cell transcriptome analysis is a powerful tool for defining cell types or sub-populations within a heterogeneous bulk population. Tumor-associated microenvironment is a complex ecosystem consisting of numerous cell types that support tumor growth, angiogenesis, immune evasion, and metastasis. With the success of checkpoint inhibitors targeting the immune cell compartment, tumor microenvi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Biology
سال: 2019
ISSN: 1474-760X
DOI: 10.1186/s13059-019-1852-7